Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYKDLTGKTAIVTGSSKGIGKAIAERFGKEKMNVVVNYHSDPSGADETLEIIKQNGGKAVSVEADVSKEEGIQALLDTALEHFGTLDVMVNNSGFNGVEAMPHEMSLEDWQRVIDVNVTGTFLGAKAALNHMMKNNIKGNVLNISSVHQQIPRPVNVQYSTSKGGIKMMTETLALNYADKGIRVNAIAPGTIAT---------ESNVDTKKEESRQKQL-KKIPMKAFGKPEEVAAAAAWLVSEEASYVTGATLFVDGGMTLYPSQLE 2DTE Chain:B ((3-248)) -FSDLRDKVVIVTGASMGIGRAIAERFVDEGSKVIDLSIHDPGEA------------KYDHIECDVTNPDQVKASIDHIFKEYGSISVLVNNAGIESYGKI-ESMSMGEWRRIIDVNLFGYYYASKFAIPYMIRSR-DPSIVNISSVQASIITKNASAYVTSKHAVIGLTKSIALDYAPL-LRCNAVCPATIDTPLVRKAAELEVGSDPMRIEKKISEWGHEHPMQRIGKPQEVASAVAFLASREASFITGTCLYVDGGLSI------

General information: TITO was launched using: RESULT: Template: 2DTE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1330 -123090 -92.55 -521.57 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -92.55 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.510

(partial model without unconserved sides chains): PDB file : Tito_2DTE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DTE-query.scw PDB file : Tito_Scwrl_2DTE.pdb: