Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDITITLDRSEQADYIYQQIYQKLKKEILSRNLLPHSKVPSKRELAENLKVSVNSVNSAYQQLLAEGYLYAIERKGFFVEELDMFSAEEHPPFALPDDLKEIHIDQSDWISFSHMSSDTDHFPIKSWFRCEQKAASRSYRTLGDMSHPQGIYEVRAAITRLISLTRGVKCRPEQMIIGAGTQVLMQLLTE-LLPKEAVYAMEEPGYRRMYQLLKNAGKQVKTIMLDEKGMSIAEIT----RQQPDVLVTTPSHQFPSGTIMPVSRRIQLLNWAAEEPRRYIIEDDYDSEFTYDVDSIPALQSLD---RFQNVIYMGTFSKSLLPGLRISYMVLPPELLRAY-KQRGYDLQTCSSLTQLTLQEFIESGEYQKHIKKMKQHYKEKRERLITALEAEFSG-EVTVKGANAGLHFVTEFDTRRTEQDILSHAAGLQLEIFGMSRFNLKENKRQTGRPALIIGFARLKEEDIQEGVQRLFKAVYGHKKIPVTGD 2ZYJ Chain:A ((30-395)) --------------------------------------------------------------------------------------------------------QRPGILSFAGGLPAPELFPKEEAAEAAARILREKGEVALQYSPTEGYAPLRAFVAEWI----G--VRPEEVLITTGSQQALDLVGKVFLDEGSPVLLEAPSYMGAIQAFRLQGPRFLTVPAGEEGPDLDALEEVLKRERPRFLYLIPSFQNPTGGLTPLPARKRLLQMVME-RGLVVVEDDAYRELYFGEARLPSLFELAREAGYPGVIYLGSFSKVLSPGLRVAFAVAHPEALQKLVQAKQGADLHTPMLNQMLVHELLKEG-FSERLERVRRVYREKAQAMLHAL-DREVPKEVRYTRPKGGMFVWMELPKGLSAEGLFRRALEENVAFVPGGPFFANGG----GENTLRLSYATLDREGIAEGVRRLGRALKGLLA------

General information: TITO was launched using: RESULT: Template: 2ZYJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2017 -208719 -103.48 -586.29 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -103.48 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.552

(partial model without unconserved sides chains): PDB file : Tito_2ZYJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZYJ-query.scw PDB file : Tito_Scwrl_2ZYJ.pdb: