Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFAVIVLPGSNC--------DIDMYHAVKDELGHEVEYVWHEET------SLDGFDGVLIPGGFSYGDYLRCGAIARFANIMPAVK--QAAAEGKPVLGVCNGFQILQELGLLPGAMRRNKDLKFICRP-VELIVQN--DETLFTASYEK-------GESITIPVAHGEGNFYCDDETLATLKENNQIAFTYGSNINGSVSDIAGVVNEKGNVLGMMPHPERAVDELLGS------A---DGLKLFQSIVKNWRETHVTTA 4L8F Chain:C ((31-278)) PIIGVLAQDVFDPKPDRNSYIAASYVKFLESA-GARVVPVMINKSEDEYSRLFKSINGVLFPGGGVSLESSGYS--KAAGIFYRLALEANSNGDYFPVWGTALGFELLTLLT---SGELLLSHT-NTSGIALPLDFTEDVKGSRLFKEFPEELMKSLATEPLTEN-SHQWSITTENFTANKKL-KKFYRVLST--NTDGYNKFVSTMEAYDFPIYATQWHPEKNAFEWTRPYIPHTPSAIKTTFYMANFFVNEARKNLH---

General information: TITO was launched using: RESULT: Template: 4L8F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1193 -93373 -78.27 -438.37 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain C : 0.65 3D Compatibility (PKB) : -78.27 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.346

(partial model without unconserved sides chains): PDB file : Tito_4L8F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4L8F-query.scw PDB file : Tito_Scwrl_4L8F.pdb: