Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFHISTLDQIKIAYIGGGSQGWARSLMSD--LSIDERMSG-TVALYDLDFEAAQKNEVIGNHSGN--GR-WRYEAVSTLKKALSAADIVIISILPGSLDDMEVDVHLPERCGIYQSVGDTVGPGGIIRGLRAVPIFAEIARAIRDYAPESWVINYTNPMSVCTRVLYKVFPGIKAIGCCHEVFGTQKLLAEMVTERLGIEVP-RREDIRVNVLGINHFTWITKASYRH-IDLLPIFREFSAHYGESGYELEGECWRDSVFCSAHRVAFDLF-ETYGAIPAAGDRHLAEFLPGPYLKQ---PEVWKFHLTPISFRKQDRAEK-RQ-ETERLIVQ-----QR--GVAEKASGEEGVNIIAALLGLGELVTNVNMPNQGQVLNLPIQAIVETNAFITRNRVQPILSGALPKGVEMLAARHISNQEAVADAGLTKDTGLAFQAFLNDPLVQIDRSDAEQLFNDMLQCIMQS 1U8X Chain:X ((26-458)) ------KKSFSIVIAGGGSTFTP--GIVLMLLDHLEEFPIRKLKLYDNDKERQDRIAGACDVFIREKAPDIEFAATTDPEEAFTDVDFVMAHIRVGKYAMRALDEQIPLKYGVV--GQETCGPGGIAYGMRSIGGVLEILDYMEKYSPDAWMLNYSNPAAIVAEATRRLRPNSKILNICDMPVGIEDRMAQIL------GLSSR-KEMKVRYYGLNHFGWWTSIQDQEGNDLMPKLKEHVSQYGYIPKT----SW-----NDTFAKARDVQAADPDTLPN-------TYLQ-YYLFPDDMVKKSN---PN-HTRANEVMEGREAFIFSQCDMITREQSSENSEIKIDDHASYIVDLARAIAYNTGERMLLIVENNGAIANFDPTAMVEVPCIVGSNGPEPITVGTIPQFQKGLMEQQVSVEKLTVEAWAEKSFQKLWQALILSKTVP-NARVARLILEDLVEANK--

General information: TITO was launched using: RESULT: Template: 1U8X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 2313 -215373 -93.11 -531.79 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain X : 0.70 3D Compatibility (PKB) : -93.11 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.495

(partial model without unconserved sides chains): PDB file : Tito_1U8X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U8X-query.scw PDB file : Tito_Scwrl_1U8X.pdb: