Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METFKSILHEAQRIVVLTGAGMSTESGIPDFRSAGGIWTEDASRMEAMSLDYFLSYPRLFWPKFKELFQMKMSGSFEPNEGHLLLAELEKQGKQVDIFTQNIDGLHKKAGSRHVYELHGSIQTAACPACGARYDLPHLLE-REVPECTAAGNNGDICGTVLKTDVVLFGDAVMH--FDTLYEKLDQADLLLVIGTSLEVAPARFVPEDASLIPGMKKVIINLEP--TYCDSLFDMVIHQKIGEFARSLGMKK 1M2H Chain:A ((3-232)) -EKLLKTIAESKYLVALTGAGVAAESGIPTFRGKDGLWNRYRPE-ELANPQAFAKDPEKVWKWYA--WRMEKVFNAQPNKAHQAFAELERLGVLKCLITQNVDDLHERAGSRNVIHLHGSLRVVRCTSCNNSFEVESAPKIPPLPKC-------DKCGSLLRPGVVWFGEMLPPDVLDRAMREVERADVIIVAGTSAVVQPAASLP---LIVKQRGGAIIEINPDETPLTPIADYSLRGKAGEV--------

General information: TITO was launched using: RESULT: Template: 1M2H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1187 -122109 -102.87 -542.71 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -102.87 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.418

(partial model without unconserved sides chains): PDB file : Tito_1M2H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1M2H-query.scw PDB file : Tito_Scwrl_1M2H.pdb: