Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLIEHYVALVKTGCAEYGQMNEMTLTEIADCLFCTERNAKLILHKLENSNWIIRESGAGRGRKSKIAFLRRPEELLLQTAKEYTMSGKLKKAKELLQQYQSAFPGLQNEYNMWLSEVFGFVTETGENGAKDVLRLFITPESVSSLDPCQIFLRSEGHFVKQIFDTLFTFDSDMQEPKPHLVHGWEEVGKKQWRFFLRKGVLFHNGQPLTSRDVAFTFQRFLELADNPYKWLLHGVKQVLEKGPYCVELILDKPNALLPYALCDERLSILPAEQG---GGKNGTGPFQMNQQH-SGMLVLEANERYFKGRPYLDRVEFVFSEQAGE-MN---GFTIQ-E-----------KQTCPEQQTVFDERHVQYLSLNLKKKGPLQHRSFRKALRLLISSERLVREAGGHRRIPVTSFLHPSP--------FEWEGVSPSELLKKSGYE--------GETIVL--YTFS-ETDHREDAEWIQNICAQHGIRLTLQFCDAADLRRPEIVQMADIIHDSATFY-QDSEFGFLHL-LLSENS-FLYQHLSEKLTQICSGMTERMFSMPDRCSRINILRDIDRQMIQELNAIPLYQNVLQVTSS-KNVKGLVLDEEGWIDLYSVWLSK 3T66 Chain:A ((12-487)) ----------------------------------------------------------------------------------------------------------------------------------EKHIHFLF-NVSTNSLDPHVDM--TYIPVRAGITETLVRVDEENVTIAPWLAESWDSTDGQHWTIKLREDVTFQNGKEMDAEAVKASLERALDES-V-AIENALKIDEIEA-DGYTLHITTKEPFPEFISELVNPNVSIIDVTEEDFTNHPVGTGPFALESFTPGSKLELVRYDEYWDGASKLDSVTFSFNEDASARSLALESGQADIVYRPEVESIETLQANEGIMVEATETFRTHNLTMNLDR-DSLKDVNVRRAVDVLLDRQEIVDTIMLGYAEVADGPFIPTLPFAPSYEKKETGTDIAIQYLEEAGYTLENQMQKDGEPLHFTVLTYGSRAELPLIAQVFQSNAKQIGIEVEIRQIEVPEEYMA-SNRDWDLITYSNVTSPRGDAGYYLNATYHPTGALNFSSVND----PELTGIIDELNRTVDQDVRAKLTEQAAAYIDEQKIHSFLIHPSAVVAYDENKVKNWVTTRSE-YYMITNQLDV

General information: TITO was launched using: RESULT: Template: 3T66.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2306 -188577 -81.78 -436.52 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -81.78 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.385

(partial model without unconserved sides chains): PDB file : Tito_3T66.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T66-query.scw PDB file : Tito_Scwrl_3T66.pdb: