Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKLKIGITC---YP-SVGGSGIIATELGKQLAEKGHEIHFITSSIPFRLNTYHPN---------I--HFH---EVEVNQYA--VFK----Y-PPYD----------LTLASKIAEVAE-RENLDIIHAHYALPHAVCAYLAKQMLKRNIGIVTTLHGTDITVLGY---D-PS--------LKDLIRFAIESSDRVTAVSSALAAETYDLIK-PEKKIETIYNFIDERVYLKKNT--------AAIKEKHGILPDEKVVIHVSNFRK-VKRVQDVIRVFRNIAG---KTKAKLLLVGDGPEKSTACELIRKYGL-EDQVL-MLGNQD--RVEDLYSISDLKLLLSEKESFGLVLLEAMACGVPCIGTNIGGIPEVIKNNVSGFLVDVGDVTAATARAMSILED-EQLSNRFTKAAIEMLENEFSSKKIVSQYEQIYADLAEPE 3FRO Chain:A ((1-432)) -RHMKVLLLGFEFLPVKVGGLAEALTAISEALASLGHEVLVFTPSHGRFQGEEIGKIRVFGEEVQVKVSYEERGNLRIYRIGGGLLDSEDVYGPGWDGLIRKAVTFGRASVLLLNDLLREEPLPDVVHFHDWHTV-FAGALIKK--YFKIPAVFTIHRLNKSKLPAFYFHEAGLSELAPYPDIDPEHTGGYIADIVTTVSRGYLIDEWGFFRNFEGKITYVFNGIDCSFWNESYLTGSRDERKKSLLSKFG-MDEGVTFMFIGRFDRGQKGVDVLLKAIEILSSKKEFQEMRFIIIGKGDPE--LEGWARSLEEKHGNVKVITEMLSREFVRELYGSVDFVIIPSYFEPFGLVALEAMCLGAIPIASAVGGLRDIITN-ETGILVKAGDPGELANAILKALELSRSDLSKFRENCKKRA-MSFSWEKSAERYVKAYTGSID--

General information: TITO was launched using: RESULT: Template: 3FRO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1964 -4390 -2.23 -11.99 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -2.23 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_3FRO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FRO-query.scw PDB file : Tito_Scwrl_3FRO.pdb: