Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHLLNPKAREIEISGIRKFSNLVAQHEDVISLTIGQPDFFTPHHVKAAAKKAIDE--NVTSYTPNAGYLELRQAVQLYMKKKADFNYDA-ESEIIITTGASQAIDAAFRTILSPGDEVIMPGPIYPGYEPIINLCGAKPVIVDTTS-----HGFKLTARLIEDALTPNTKCVVLPYPSNPTGVTLSEEELKSIAALLKGRNVFVLSDEIYSELTYDRPH-YSIATYL--RDQTIVINGLSKSHSMTGWRIGFLFAPKDIAKHILKVHQYNVSCASSISQKAALEAVTNGF-------DDALIMREQYKKRLDYVYDRLVSMGLDVVKPSGAFYIFPSIKSFG------------MTSFDFSMALLEDAGVALVPGSSFSTY-----GEGYVRLSFACSMDTLREGLDRLELFVLKKREAMQTINNGV 4WP0 Chain:A ((6-424)) ----QARRLDGIDYNPWVEFV-KLASEHDVVNLGQGFPDFPPPDFAVEAFQHAVSGDFMLNQYTKTFGYPPLTKILASFFGELLGQEIDPLR-NVLVTVGGYGALFTAFQALVDEGDEVIIIEPFFDCYEPMTMMAGGRPVFVSLKPGLGSSSNWQLDPMELAGKFTSRTKALVLNTPNNPLGKVFSREELELVASLCQQHDVVCITDEVYQWMVYDGHQHISIASLPGMWERTLTIGSAG-TFSATGWKVGWVLGPDHIMKHLRTVHQNSVFHCPTQSQAAVAESFEREQLLFRQPSSYFVQFPQAMQRCRDHMIRSLQSVGLKPIIPQGSYFLITDISDFKRKMPDLPGAVDEPYDRRFVKWMIKNKGLVAIPVSIFYSVPHQKHFDHYIRFCFVKDEATLQAMDEKLRKWKVELEN---------

General information: TITO was launched using: RESULT: Template: 4WP0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2098 -258054 -123.00 -684.49 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -123.00 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.614

(partial model without unconserved sides chains): PDB file : Tito_4WP0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WP0-query.scw PDB file : Tito_Scwrl_4WP0.pdb: