Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLRQPMPELTGEKAWLNGE-VTREQLIGEKPTLIHFWSISCHLCKEAMPQVNEFRDKYQD-QLNVVAVHMPRSEDDLDPGKIKETAAEHDITQPIFVDSDHALTDAFENEYVPAYYVFDKTGQLRHFQAGGSGMKMLEKRVNRVLAETE 2L5O Chain:A ((1-139)) --DSKTAPAFSLPD--LHGKTVSNADL-QGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPI----DPIESVRQYVKDYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAWRN--

General information: TITO was launched using: RESULT: Template: 2L5O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 649 -79464 -122.44 -580.03 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -122.44 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.686

(partial model without unconserved sides chains): PDB file : Tito_2L5O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2L5O-query.scw PDB file : Tito_Scwrl_2L5O.pdb: