Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLPLHISLEKKKVVIAGGGSIALRRLKTVISEGADITLVSPDVEPE-IKQMAEERRIKWEKRTIEKEDYLNAFFIIAATDNAAVNKEIAQSASP-FQLVNCVSDAELGNVYMPKIVKRGHVTVSVS-TSGASPKHTKELAENVDKLIDGDFVAEVNRLYQMRRKK 2X5O Chain:A ((2-125)) ------DYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGLDKLPE--AVERHTGSLNDEWLMAADLIVASPGIAL-AHPSLSAAADAGIEIVG--DIELF----CR-EAQ-APIVAITGSNGKSTV-TTLVGEMAK---------------------

General information: TITO was launched using: RESULT: Template: 2X5O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 474 -64673 -136.44 -534.49 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -136.44 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.602

(partial model without unconserved sides chains): PDB file : Tito_2X5O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2X5O-query.scw PDB file : Tito_Scwrl_2X5O.pdb: