Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHRGKGMKFVGDSRIPAEKKPNIPKDYSEYPGKTEAFWPNFLLKEWMVGAVFLIGFLVLTIVHQPPLERMADPTDTGYIPLPDWYFLFLYQLLKYEYAAGSFTVVGAMIMPGLAFGALLLAPFLDRGTERRPWKRPVAVGMMLLAISAAVFLTWQSVATHDWAKAEEQGKITKEADIDTNAEGYKVFKEQG---CISCHGDNLQGG--AAGPSLVDSGLKPDEIKKIAVEGKGKMPAGVFKGNDKQLEELAKFISETTAK--- 4PWA Chain:A ((3-88)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EDKLALGREIFLERSEPQCALCHTLADAEAVGEVGPNLDELKPDAERVNTAVTNGIGPMPA-NEILTDEEIEAVALYVSTVAGKAKN

General information: TITO was launched using: RESULT: Template: 4PWA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 321 7486 23.32 95.97 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : 23.32 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.588

(partial model without unconserved sides chains): PDB file : Tito_4PWA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PWA-query.scw PDB file : Tito_Scwrl_4PWA.pdb: