Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEYYMKLALDLAKQGEGQTESNPLVGAVVVKDGQIVGMGAHLKYGEAHAEVHAIHMAGAH---AEGADIYVTLEPCSHYGKTPPCAELIINSGIKRVFVAMRDPNPLVAGRGISMMKEAGIEVREGILADQAERLNEKFLHFMRTGLPYVTLKAAASLDGKIATSTGDSKWITSEAARQDAQQYRKTHQSILVGVGTVKADNPSLTCRLPNVTKQPVRVILDTVLSIPED-AKVICDQIAPTWIFTTARADEEKKKRLSAFGVNIFTLETERIQIPDVLKILAEEGIMSVYVEGGSAVHGSFVKEGCFQEIIFYFAPKLIGGTHAPSLISGEGFQSMKDVPLLQFTDITQIGRDIKLTAKPTKE 2HXV Chain:A ((13-357)) MYETFMKRAIELAKKGLGRVNPNPPVGAVVVKDGRIIAEGFHPYFGGPHAERMAIESARKKGEDLRGATLIVTLEPCDHHGKTPPCTDLIIESGIKTVVIGTRDPNPV-SGNGVEKFRNHGIEVIEGVLEEEVKKLCEFFITYVTKKRPFVALKYASTLDGKIADHRGDSKWITDK-LRFKVHEMRNIYSAVLVGAGTVLKDNPQLTCRLKE-GRNPVRVILDRKGVLSGKVFRVFEE-NARVIVFTESEEAE------YPPHVEKAL---SDCSVESILRNLYERDIDSVLVEGGSKVFSEFLDH--ADVVFGFYSTKIFGK-GLD--VFSGYLSDVSVPPKFKVVNVEFSDSEFLVEMRPC--

General information: TITO was launched using: RESULT: Template: 2HXV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1937 -16357 -8.44 -47.97 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -8.44 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_2HXV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HXV-query.scw PDB file : Tito_Scwrl_2HXV.pdb: