Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGNTRKKVSVIGAGFTGATTAFLIAQKELA-DVVLVDIPQLENPTKGKALDMLEASPVQGFDAKITGTSNYEDTAGSDIVVITAGIARKPGMSRDDLVSTNEKIMRSVTQEIVKYSPDSIIVVLTNPVDAMTYAVYKESGFPKERVIGQSGVLDTARFRTFVAEELNLSVKDVTGFVLGGHGDDMVPLVRYSYAGGIPLETLIPKERIDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTEMVEAILKDQRRVLPTIAYLEGEYGYEGIYLGVPTIVGGNGLEQIIELELTDYERAQLNKSVESVKNVMKVLS 1GUZ Chain:D ((2-305)) ------KITVIGAGNVGATTAFRLAEKQLARELVLLDV--VEGIPQGKALDMYESGPVGLFDTKVTGSNDYADTANSDIVIITAGL----------LLMKNAGIVKEVTDNIMKHSKNPIIIVVSNPLDIMTHVAWVRSGLPKERVIGMAGVLDAARFRSFIAMELGVSMQDINACVLGGHGDAMVPVVKYTTVAGIPISDLLPAETIDKLVERTRNGGAEIVEHLKQGSAFYAPASSVVEMVESIVLDRKRVLPCAVGLEGQYGIDKTFVGVPVKLGRNGVEQIYEINLDQADLDLLQKSAKIVDENCKML-

General information: TITO was launched using: RESULT: Template: 1GUZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1708 -101414 -59.38 -346.12 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain D : 0.87 3D Compatibility (PKB) : -59.38 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_1GUZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GUZ-query.scw PDB file : Tito_Scwrl_1GUZ.pdb: