Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHKKIYLSPPHMSGREQHYISEAFRSNWIAPLGPLVNSFEEQLAERVGVKAAAAVGSGTAAIHLALRLLEVKEGDSVFCQSFTFVATANPILYEKAVPVFIDSEPDTWNMSPTALERALEEAKRNGTLPKAVIAVNLYGQSAKMDEIVSLCDAYGVPVIEDAAESLGTVYKGKQSGTFGRFGIFSFNGNKIITTSGGGMLVSNDEAAIEKARFLASQAREPAV--HYQHSEIGHNYRLSNILAGVGIAQLEVLDERVEKRRTIFTRYKNALGHLD----GVRFMPEYAAGVSNRWLTTLTLDNG---LSPYDIVQRLAEENIEARPLWKPLHTQPLFDPALFYSHEDTGSVCEDLFKRGICLPSGSNMTEDEQGRVIEVLLHLFHTVEVKKWTASIR 4LC3 Chain:A ((14-389)) -APFLPFTRPEIDEETIQGVVEVLRSGWI-TTGPQCQKFEAALSEYCGGRPVRVFNSGTCTLEIGLRIAGVGPGDEVITTPASWVSTSNVIIETGATPVFADIDPVTRNIDLDKLEQAIT------PRTKAIIPVFLSGLPVDMDRLYAIARAHKLRVIEDAAQAFGSTWHGKRIGAIGDLVSFSFHANKNLTTIEGGALVLNNEDEAVLAQKYRLQGITRTGFDGMDCDVLGGKYNLTDVAARVGLGQLPHLERFTAQRRALARAYFAAFDGGAAAKLGVGLPVAE-FENGNWHMFLVTLPLERLTITRAEFMAQMKERGIGTGIHYPAIHLFTLYRARGF--KEGMFPHAERYGASTVTLPLFTQMTEGDVRRVVDAVNQICEQY----------

General information: TITO was launched using: RESULT: Template: 4LC3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2103 -116689 -55.49 -317.95 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -55.49 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_4LC3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LC3-query.scw PDB file : Tito_Scwrl_4LC3.pdb: