Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNTIETILNHRSIRSFT-DQLLTAEEIDTLVKSAQAASTSSYVQAYSIIGVSDPEKKRELSVLAGNQPYVEKNGHFFVFCADLYRHQQLAEEKGEHISE--LLENTEMFMVSLIDAALAAQNMSIAAESMGLGICYIGGIRNELDKVTEVLQT-PDHVLPLFGLAVGHP--ANLSGKKPRLPKQAVYHENTYNVNTDDFRHTMNTYDKTISDYYRERTNGKREETWSDQILNFMKQKPRTYLNDYVKEKGFNKN 1BKJ Chain:A ((2-240)) -NNTIETILAHRSIRKFTA-VPITDEQRQTIIQAGLAASSSSMLQVVSIVRVTDSEKRNELAQFAGNQAYVESAAEFLVFCIDYQRHATINP--------DVQADFTELTLIGAVDSGIMAQNCLLAAESMGLGGVYIGGLRNSAAQVDELLG-LPENSAVLFGMCLGHPDQ--NPEVKPRLPAHVVVHENQYQELN---LDDIQSYDQTMQAYY---------STWSQEVTGKLAGESRPHILPYLNSKGLAKR

General information: TITO was launched using: RESULT: Template: 1BKJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 868 -29085 -33.51 -129.84 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -33.51 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_1BKJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BKJ-query.scw PDB file : Tito_Scwrl_1BKJ.pdb: