Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASSSGSKAEFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFC-SRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGI-GRHCNKLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQAQTPTGKQTDKTKSNMKGF 4NFN Chain:A ((15-301)) --------ANYVVKDRWKVLKKIGGGGFGEIYEAMDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNEKFNYVVMQLQGRNLADLRRSQPRGTFTLSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTYRKCYMLDFGLARQYTNTT-GDVRPPRNVAGFRGTVRYASVNAHKNREMGRHDDLWSLFYMLVEFAVGQLPWRKIKD---KEQVGMIKEKYE---HRMLLKHMPSEFHLFLDHIASLDYFTKPDYQLIMSVFENSMKERGIAENEAFDWEK-------------------------------------

General information: TITO was launched using: RESULT: Template: 4NFN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -151063 for 2274 contacts (-66.4/contact) + 2D Compatibility (PS) -30158 + (NN) -11986 + (LL) 2020 1D Compatibility (HY) -22800 + (ID) 5100 Total energy: -219087.0 ( -96.34 by residue) QMean score : 0.383

(partial model without unconserved sides chains): PDB file : Tito_4NFN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NFN-query.scw PDB file : Tito_Scwrl_4NFN.pdb: