Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------MTHVKALAIKGIMTIIVLYLVLGLGFGFTFGDTLLMTIVLGVIS-------------YLLGDLYVLPKWNN--MIATLADFGLAFLVIWLMGMPLSMGMTGGEVALAALFGAIVMAIGEYCFHFYMMSKEIGKK-HYLETRTDS 2PR1 Chain:A ((4-155)) MTKVERLLINYKTLEEFKKFKEYGIQELSMLEELQDNIIENDSTSPFYGIYFGDKLVARMSLYQVNGKSNPYFDNRQDYLELWKLEVLPGYQNRGYGRALVEFAKSF------KMPIRTNPRMKSAEFWNKMNFKTVK--------YDMARDKGEDPLIWHPDMDR

General information: TITO was launched using: RESULT: Template: 2PR1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 354 -65777 -185.81 -576.99 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -185.81 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.109

(partial model without unconserved sides chains): PDB file : Tito_2PR1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PR1-query.scw PDB file : Tito_Scwrl_2PR1.pdb: