Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MQKLSEEMKQTIMDIFEHLHANPEVSWKE------------YETTSFLKQKLEDLGCRTRTFSDCTGVVGEIGSGSPVVAVRADIDAL-----WQ--------EVNGTFRANHSCGHDSHMTMALGTLMLLKKQPELPKGTIRFIFQPAEEKGGGALKMIEE---------------------------------------GVLDDID--------------YLYGVHVRPIQETQNGRCAPS----ILHGSSQHIEGTIIGEEAHGARPHLGKNSIEIAAFLVHKLG--------LIHIDPQIPHTVKMTKLQAGGESSN-------------IIPGKASFSLDLRAQTNEAMEALIAETERACEAAAAAFGAKIELHKEHSLPAATQNKEAEAIMAEAITEIIGAERLDDPLVTTGGEDFHFYAVKVPNLKTTMLGLGCGLQPGLHHPHMTFDRNAMFTGIHILANAVLKTFQKAESLAAANAS 1LFW Chain:A ((1-468)) MDLNFKELAEAKKDAILKDLEELIAIDSSEDLENATEEYPVGKGPVDAMTKFLSFAKRDGFDTENFANYAGRVNF-GAGDKRLGIIGHMDVVPAGEGWTRDPFKMEIDEEGRIYGRGSADDKGPSLTAYYGMLLLKEAGFKPKKKIDFVLGTNEETNWVGIDYYLKHEPTPDIVFSPDAEYPIINGEQGIFTLEFSFKNDDTKGDYVLDKFKAGIATNVTPQVTRATISGPDLEAVKLAYESFLADKELDGSFEINDESADIVLIGQGAHASAPQVGKNSATFLALFLDQYAFAGRDKNFLHFLAEVEHEDFYGKKLGIFHHDDLMGDLASSPSMFDYEHAGKASLLNNVRYPQGTDPDTMIKQVLDKFSG-----ILDVTYNGFEEPHYVPGSDPMVQTLLKVYEKQTGKPG---HEVVIGG---GTYGRLFERG-VAFGAQPENGPMVMHAANEFMMLDDLILSIAIYAEAIYELTKDE---------

General information: TITO was launched using: RESULT: Template: 1LFW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1759 -149566 -85.03 -414.31 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -85.03 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.385

(partial model without unconserved sides chains): PDB file : Tito_1LFW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1LFW-query.scw PDB file : Tito_Scwrl_1LFW.pdb: