Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQLFKSFDASEKTQLICFPFAGGYSASFRPLHAFLQGECEMLAAEPPGHGTNQTSAIEDLEELTDLYKQELNLRPDRPFVLFGHSMGGMITFRLAQKLEREGIFPQAVIISAIQPPHIQ-R---KK---VSHLPD-DQ-FLDHIIQLGGMPAELVENKEVMSFFLPSF-----RSDYR-ALEQFE----LYDLAQIQSPVHVFNGLDDKK-CIRDAEGWKKWAKDITFHQFD-GGHMFLLSQTEEVAERIFAILNQHPIIQP 3BF7 Chain:A ((14-254)) ----------HNNSPIVLVHGLFGSLDNLGVLARDLVNDHNIIQVDVRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALAPD---RIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAI--M-RQHLNEEGVIQFLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLLAQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND------

General information: TITO was launched using: RESULT: Template: 3BF7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1179 -156645 -132.86 -712.02 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -132.86 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.513

(partial model without unconserved sides chains): PDB file : Tito_3BF7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3BF7-query.scw PDB file : Tito_Scwrl_3BF7.pdb: