Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASIAVCPGSFDPVTYGHLDIIKRGAHIFEQVYVCVLNNSSKKPLFSVEERCELLREVTKDIPNITVETSQGLLIDYAKRKNAKAILRGLRAVSDFEYEMQGTSVNRVLDESIETFFMMTNNQYSFLSSSIVKEVARYNGSVSEFVPPEVELALQQKFRQG 3LCJ Chain:A ((4-156)) ----AVCPGSFDPVTLGHVDIFERAAAQFDEVVVAILVNPAKTGMFDLDERIAMVKESTTHLPNLRVQVGHGLVVDFVRSCGMTAIVKGLRTGTDFEYELQMAQMNKHI-AGVDTFFVATAPRYSFVSSSLAKEVAMLGGDVSELLPEPVNRRLRDRL---

General information: TITO was launched using: RESULT: Template: 3LCJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 733 -111398 -151.97 -728.09 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -151.97 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.406

(partial model without unconserved sides chains): PDB file : Tito_3LCJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LCJ-query.scw PDB file : Tito_Scwrl_3LCJ.pdb: