Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRQITDISQLKEAIKQYHSEGKSIGFVPTMGFLHEGHLTLADKA-RQENDAVIMSIFVNPAQFGPNEDFEAYPRDIERDAALAENAGVDILFTPDAHDMYPGEKNVTIHVERRTDVLCGRSREGHFDGVAIVLTKLFNLVKPTRAYFGLKDAQQVAVVDGLISDFFMDIELVPVDTVREEDGLAKSSRNVYLTAEERKEAPKLYRALQTSAELVQAGERDPEAVIKAAKDIIETTSGT-IDYVELYSYPELEPVNEIAGKMILAVAVAFSKARLIDNIIIDIREMERI 2A86 Chain:A ((19-287)) -----DVADVSRALRL---TGRRVMLVPTMGALHEGHLALVRAAKRVPGSVVVVSIFVNPMQFGAGGDLDAYPRTPDDDLAQLRAEGVEIAFTPTTAAMYPDGLRTTVQPGPLAAELEGGPRPTHFAGVLTVVLKLLQIVRPDRVFFGEKDYQQLVLIRQLVADFNLDVAVVGVPTVREADGLAMSSRNRYLDPAQRAAAVALSAALTAAAHAATAGA---QAALDAARAVLDAAPGVAVDYLELRDI-GLGPM-PLNGSGRLLVAARLGTTRLLDNIAIEI------

General information: TITO was launched using: RESULT: Template: 2A86.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1371 -82030 -59.83 -307.23 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -59.83 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_2A86.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A86-query.scw PDB file : Tito_Scwrl_2A86.pdb: