Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSWIVNKQSSQEELAGRFRKLMSAPDILQIPGAHDGMAALLAKEAGFSAIYLSGA-AYTASRGLPDLGIITSAEIAERAKDLVRAADLPLLVDIDTGFGGVLNAARTAREMLEARVAAVQMEDQQLPKKCGHLNGKQLVPIKEMAQKIKAIKQAA--PSLIVVARTDARAQEGLDAAIKRSEAYIEAGADAIFPEALQAENEFRQFAERIPVPLLANMTEFGKTPYYRAD-EFEDMGFHMVIYPVTSLRAAAKACERMFGLMKEHGSQKEGLHDMQTRKELYDTISYYDYEALDKTIAKTVLPDE 3B8I Chain:C ((3-248)) -------RASHHELRAMFRALLDSSRCYHTASVFDPMSARIAADLGFECGILGGSVASLQVLAAPDFALITLSEFVEQATRIGRVARLPVIADADHGYGNALNVMRTVVELERAGIAALTIEDTLLPAQFGR-KSTDLICVEEGVGKIRAALEARVDPALTIIARTNAELID-VDAVIQRTLAYQEAGADGICLVGVRDFAHLEAIAEHLHIPLM--LVTYGN-PQLRDDARLARLGVRVVVNGHAAYFAAIKA---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3B8I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1306 -65302 -50.00 -274.38 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain C : 0.75 3D Compatibility (PKB) : -50.00 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.618

(partial model without unconserved sides chains): PDB file : Tito_3B8I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3B8I-query.scw PDB file : Tito_Scwrl_3B8I.pdb: