Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGNTRKKVSVIGAGFTGATTAFLIAQKELA-DVVLVDIPQLENPTKGKALDMLEASPVQGFDAKITGTSNYEDTAGSDIVVITAGIARKPGMSRDDLVSTNEKIMRSVTQEIVKYSPDSIIVVLTNPVDAMTYAVYKESGFPKERVIGQSGVLDTARFRTFVAEELNLSVKDVTGFVLGGHGDDMVPLVRYSYAGGIPLETLIPKERIDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTEMVEAILKDQRRVLPTIAYLEGEYGYEGIYLGVPTIVGGNGLEQIIELELTDYERAQLNKSVESVKNVMKVLS 1GUZ Chain:C ((2-305)) ------KITVIGAGNVGATTAFRLAEKQLARELVLLDV--VEGIPQGKALDMYESGPVGLFDTKVTGSNDYADTANSDIVIITAGLPRKPGMTREDLLMKNAGIVKEVTDNIMKHSKNPIIIVVSNPLDIMTHVAWVRSGLPKERVIGMAGVLDAARFRSFIAMELGVSMQDINACVLGGHGDAMVPVVKYTTVAGIPISDLLPAETIDKLVERTRNGGAEIVEHLKQGSAFYAPASSVVEMVESIVLDRKRVLPCAVGLEGQYGIDKTFVGVPVKLGRNGVEQIYEINLDQADLDLLQKSAKIVDENCKML-

General information: TITO was launched using: RESULT: Template: 1GUZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1734 -89760 -51.76 -296.24 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain C : 0.88 3D Compatibility (PKB) : -51.76 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.553

(partial model without unconserved sides chains): PDB file : Tito_1GUZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GUZ-query.scw PDB file : Tito_Scwrl_1GUZ.pdb: