Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNTELELLRQKADELNLQILKLINERGNVVKEIGKAKEAQGVNRFDPVRERTMLNNIIENNDGPFENSTIQHIFKEIFKAGLELQEEDHSKALLVSRKKKPEDTIVDIKGEKIGDGQQRFIVGPCAVESYEQVAEVAAAAKK-----QGIKILRGGAFKP--RTSPYDFQGLGVE-GLQILKRVADEFDLAVISEIVTPAHIEEALDYIDVIQIGARNMQNFELLKAAGAVKKPVLLKRGLAATISEFINAAEYIMSQGNDQIILCERGIRTYETATRNTL--DISAVPILKQETHLPVFVDVTHST----GRRDLLLPTAKAALAIGADGVMAEVHPDPSVALSDSAQQMAIPEFEKWLNELKPMVKVNA 1FWT Chain:B ((7-244)) -------------------------------------------------------------------------------------------------------------------------IAGPCAIESEELLLKVGEEIKRLSEKFKEVEFVFKSSFDKANRSSIHSFRGHGLEYGVKALRKVKEEFGLKITTDIHESWQAEPVAEVADIIQIPAFLCRQTDLLLAAAKTGRAVNVKKGQFLAPWDTKNVVEKLKFGGAKEIYLTERGT----TFGYNNLVVDFRSLPIMKQWAK--VIYDATHSVQLPG-MREFIFPLIRAAVAVGCDGVFMETHPEPEKALSDASTQLPLSQLE--------------

General information: TITO was launched using: RESULT: Template: 1FWT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1159 15026 12.96 69.56 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : 12.96 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_1FWT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FWT-query.scw PDB file : Tito_Scwrl_1FWT.pdb: