Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVKMKKCSREDLQTLQQLSIETFNDTFKEQ----N----SPENMKAYLESAFNTEQLEKELSNMSSQFFFIYFDHEIAGYVKVNIDDAQSEEMGAESLEIERIYIKNSFQKHGLGKHLLNKAIEIALERNKKNIWLGVWEKNENAIAFYKKMGFVQTGAHSFYM---GDEEQTDLIMAKTLI 1GHE Chain:A ((4-173)) --AQLRRVTAESFAHYRHGLAQLLFETVHGGASVGFMADLDMQQAYAWC------DGLKADIAAGSLLLWVVAEDDNVLASAQLSLCQKP---NGLNRAEVQKLMVLPSARGRGLGRQLMDEVEQVAVKHKRGLLHLDTE-AGSVAEAFYSALAYTRVGELPGYCATPDGRLHPTAIYFKTL-

General information: TITO was launched using: RESULT: Template: 1GHE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 677 -3886 -5.74 -24.44 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -5.74 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_1GHE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GHE-query.scw PDB file : Tito_Scwrl_1GHE.pdb: