Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFMFEKPHGMRDTLPGLYETKKKVRRSLTDLIDKWGYQFMETPTLEFYDTVGVQS--AIEEQQLFKLLDQDGKTLVLRPDMTGPIARVAASKLL-KHGHPLRVGYAANVFRAQEREGGRPAEFEQVGVELIGDGTTSADAEVIALVVGALKNAGLASFKIAIGHAGIADALFVEVLGNVERADVLRRFLYEKNYVGYREH--VKSLPLSSIDKSRLLELLELRGGI--EVCGRA--EEIVDSAQGKSVVDELKALWDILEDYGCTENVRLDLNMVSHMSYYTGILFEVYAENVGFVIGSGGRYNKLLGHFDS-PAPATGFGLRIDRLIEALHMKDEP---C-EIDAV-IFSKEQRAQ-AIAYANEERMKGNKVVLQDL-SGIE-NIDQMTKSFANVTYFIGARKEEQNG 1H4V Chain:B ((2-391)) --TARAVRGTKDLFGKELRMHQRIVATARKVLEAAGALELVTPIFEETQVFEKGVGA----KEMFTFQDRGGRSLTLRPEGTAAMVRAYLEHGMKVWPQPVRLWMAGPMFRAERP----YRQFHQVNYEALGSENPILDAEAVVLLYECLKELGLRRLKVKLSSVGDPEDRARY-------NAYLREVLSPH-REALSEDSKERL-------EENPMRILDSKSERDQALLKELGVRPMLD-FLGEEARAHLKEVERHLERLSV--PYELEPALVRGLDYYVRTAFEVHH----SALGGGGRYDGLSELLGGPRVPGVGFAFGVERVALALEAEGFGLPEEKGPDLYLIPLTEEAVAEAFYLAEALRPR-LRAEYALAPRKPAKGLEEALKRGAAFAGFLGEDELRA--

General information: TITO was launched using: RESULT: Template: 1H4V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1724 9188 5.33 25.81 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : 5.33 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.452

(partial model without unconserved sides chains): PDB file : Tito_1H4V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H4V-query.scw PDB file : Tito_Scwrl_1H4V.pdb: