Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQLFKSFDA-SEKTQLICFPFAGGYSASFRPLHAFLQGECEMLAAEPPGHGTNQ-TSAIEDLEELTDLYKQELNLRPDRPFVLFGHSMGGMITFRLAQK-LEREGIFPQAVIISAIQPPHIQR---KKVSHLPD----DQ-FLDHIIQL-GGMPAELVENKEVMSFFLP----SFRSDYR-ALEQFE----L-YDLA--QIQSPVHVFNGLDDKK-CIRDAEGWKKWAKDITFHQFDG-GHMFLLSQTEEVAERIFAILNQHPIIQP 4CFS Chain:A ((29-285)) ----YIDNQRDTDGPAILLLPGWCHDHRVYKYLIQELDADFRVIVPNWRGHGLSPSEVPDFGYQEQVKDALEILDQLGVETFLPVSHSHGGWVLVELLEQAGPE---RAPRGIIMDWLMWAPKPDFAKSLTLLKDPERWREGTHGLFDVWLDGHDEKRVR-HHLLEEMADYGYDCWGRSGRVIEDAYGRNGSPMQMMANLTKTRPIRHIFSQPTEPEYEKINSDFAEQHPWFSYAKLGGPTAFPAIDVPDRAAVHIREFATAIRQ---

General information: TITO was launched using: RESULT: Template: 4CFS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1205 -140722 -116.78 -609.19 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -116.78 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.510

(partial model without unconserved sides chains): PDB file : Tito_4CFS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CFS-query.scw PDB file : Tito_Scwrl_4CFS.pdb: