Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTKADNRHLYLKVIERIKEDIKNGIYTEKEKLPSEFELSKKLGVSRATLREALRILEEEHVIIRRHGVGTFVHSKPLFLSGIEQLNSVTKMIEQANMTPGTIFLSSQVLMPSEDDTKRFHLAEGQELFYLERVRTADGQPIVYCIDKIPMNILPNSFSH--QQESMFDLLEKNSGSVISYAVTDIEPIGYHDTISPVLECDPETALLLLKQTHYDQHDKPVLYSLNYFRADKFRFHVLRKRF- 3F8M Chain:A ((14-243)) ---------LKHQVVRAELDRMLDG-MRIGDPFPAEREIAEQFEVARETVRQALRELLIDG-RVERRGRTTVVARPK--IRQPLGMGSYTEAAKAQGLSAGRILVAWSDLTADEVLAGVLGVDVGAPVLQLERVLTTDGVRVGLETTKLPAQRYPGLRETFDHEASLYAEIRSR-GIAFTRTVDTIDTALPDAREAALLGADARTPMFLLNRVSYDQDDVAIEQRRSLYRGDRMTFTAVMHAKN

General information: TITO was launched using: RESULT: Template: 3F8M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1019 -72359 -71.01 -318.76 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -71.01 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.546

(partial model without unconserved sides chains): PDB file : Tito_3F8M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F8M-query.scw PDB file : Tito_Scwrl_3F8M.pdb: