Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MTTDIIKTILLVIVIIAAAAVGL----IKGDFFSADQKTSQTKE-YDETMAFPSDRYPETAKHIKDAINEGH-SEVCTIDRDGAEERREQSLKDVPSKKGYDRDEWP--------------------------MAMCKEG-GEGASVEY-ISPADNRG-----------AGSWVGHRLTDYPDGTK-VLFTIQ-------- 3KOS Chain:A ((11-210)) QEKLKIGVVGTFAIGCLFPLLSDFKRSYPHIDLHISTHNNRVDPAAEGLDYTIRYGGGAWHD---TDAQYLCSALMSPLCSPTLA-SQIQTPADILKFPLLRSYRRDEWALWMQTVGEAPPSPTHNVMVFDSSVTMLEAAQAGMGVAIAPVRMFTHLLSSERIVQPFLTQIDLGSYWITRLQSRPETPAMREFSRWLTGVLHKT

General information: TITO was launched using: RESULT: Template: 3KOS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 594 -49039 -82.56 -342.93 target 2D structure prediction score : 0.28 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -82.56 2D Compatibility (Sec. Struct. Predict.) : 0.28 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.178

(partial model without unconserved sides chains): PDB file : Tito_3KOS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KOS-query.scw PDB file : Tito_Scwrl_3KOS.pdb: