Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRHYEAVVIGGGIIGSAIAYYLAKENKNTALFESGTMGGRTTSAAAGMLGAHAECEERDAFFDFAMHSQRLYKGLGEELYALSGVDIRQHNGGMFKLAFSEEDVLQLRQMDD------LDSVSWYSKEEVLEKEPYASG-DIFGASFIQDDVHVEPYFVCKAYVKAAKMLGAEIFEHTPVLHVERDG-EALFIKTPSGDVWANHVVVASGVWSGMFFKQLGL--NNAFLPVKGECLSVWNDDIPLTKTL-Y---HDHCYIVPRKSGRLVVGATMKPG-DWSETPDLGGLESVMKKAKTMLPAIQNMKVDRFWAGLRPGTKDGKPYIGRHPEDSRILFAAGHFRNGILLAPATGALISDLIMNKEVNQDWLHAFRIDRKEAVQI 1Y56 Chain:B ((2-372)) LPEKSEIVVIGGGIVGVTIAHELAKRGEEVTVIEKRFIGSGSTFRCGTGIRQQ--FN-DEANVRVMKRSVELWKKYSEEY----G--FSFKQTGYLFLLYDDEEVKTFKRNIEIQNKFGVPT-KLITPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAVKAKEYGAKLLEYTEVKGFLIENNEIKGVKTNKGIIKTGIVVNATNAWANLINAMAGIKTKIPIEPYKHQAVITQPIKRGTINPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKNLLILRTWAGYYAKTPDSNPAIGRIEELNDYYIAAGFSGHGFMMAPAVGEMVAELITKGK-TKLPVEWYDPYRFERG--

General information: TITO was launched using: RESULT: Template: 1Y56.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2156 -227778 -105.65 -639.83 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -105.65 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.637

(partial model without unconserved sides chains): PDB file : Tito_1Y56.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Y56-query.scw PDB file : Tito_Scwrl_1Y56.pdb: