Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MGKKMTIASLILMTA-GLTACGANDNAMNDTRNNGNTRPIGYYTNEN--DADRQGDGIDHDGPVSELMEDQNDGNRNTT----NVNNRDRVTA-----DDRVPLATDGTYNNT-----NNRNMNRNAANNGYDNQE--NRRLAAKIANRVKQVKNVNDTQVMVSDDRVVIAVKSHREFTKSDRDNVVKAARNY------ANGRDVQVSTDKGLFRKLHKMNNR 4ZFL Chain:A ((1-229)) ARHVAWLGAPRSLADLVLDPPQGLLVQSYAPRRQKHGLMNADGWGAGFFDDDGVARRWRSDKPLWGDASFASVAPALRSRCVVAAVRSATIGMPIEPSASAPFSDGQWLLSHNGLVDRGVLPLTGAAESTVDSAILAALIFSRGLDALGATIAEVGELDPNARLNILAANGSRLLATTWGDTLSVLRRPDGVVLASEPYDDDPGWSDIPDRHLVDVRDAHVVVTPLLEH

General information: TITO was launched using: RESULT: Template: 4ZFL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1223 78033 63.80 394.11 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 63.80 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.264

(partial model without unconserved sides chains): PDB file : Tito_4ZFL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZFL-query.scw PDB file : Tito_Scwrl_4ZFL.pdb: