Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNHLQLKKLLNHFFLEDIGTGDLTSQSIFGEQSCEAEIVAKSEGIFAGAAIIKEGFSLLDENVQSILHKKDGDMLHKGEVIAELHGPAAALLSGERVVLNLIQRLSGIATMTREAVRCLDDEQIK--ICDTRKTTPGLRMLEKYAVRAGGGYNHRFGLYDGIMIKDNHIAACGSILEACKKARQAAGHMVNIEVEIETEEQLREAIAAGADVIMFDNCPPDTVRHFAKLTPA---NIKTEASGGITLESLPAFKGTGVNYISLGFLTHSVKSLDISMDVTLSNESVEECCYVNS 5AYY Chain:I ((10-285)) LPPVTLAALVDSWLREDC-PGLNYAALVSGAGPSQAALWAKSPGVLAGQPFFDAIFTQLNCQVSWFL--PEGSKLVPVARVAEVRGPAHCLLLGERVALNTLARCSGIASAAAAAVEAARGAGWTGHVAGTRKTTPGFRLVEKYGLLVGGAASHRYDLGGLVMVKDNHVVAAGGVEKAVRAARQAADFALKVEVECSSLQEAVQAAEAGADLVLLDNFKPEELHPTATVLKAQFPSVAVEASGGITLDNLPQFCGPHIDVISMGMLTQAAPALDFSLKL---------------

General information: TITO was launched using: RESULT: Template: 5AYY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain I - contact count / total energy / energy per contact / energy per residue : 1392 -64590 -46.40 -238.34 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain I : 0.80 3D Compatibility (PKB) : -46.40 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.587

(partial model without unconserved sides chains): PDB file : Tito_5AYY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AYY-query.scw PDB file : Tito_Scwrl_5AYY.pdb: