Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILKKILPYSKELLKMAAGEGDIVVDATMGNGHDTQFLAELVGENGHVYAFDIQESAVANTKERLGDMYQARTTLFHKSHDKIAESLPPETHGKVAAAVFNLGYLPGGDKSITTNGSSTIKAIEQLLSIMKDEGLIVLVVYHGHPEGKAEKNDVLEFCRDLDQQTARVLTYGFINQQNDPPFIVAIEKKAQISK 3MTI Chain:A ((5-181)) -----PIHMSHDFLAEVLDDESIVVDATMGNGNDTAFLAGLS---KKVYAFDVQEQALGKTSQRLSDLGIENTELILDGHENLDHY-V---REPIRAAIFNLG-----------KPHTTLEAIEKILDRLEVGGRLAIMIY------DMEKDAVLEYVIGLDQRVFTAMLYQPLNQINTPPFLVMLEKL-----

General information: TITO was launched using: RESULT: Template: 3MTI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 800 -116319 -145.40 -726.99 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -145.40 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.596

(partial model without unconserved sides chains): PDB file : Tito_3MTI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MTI-query.scw PDB file : Tito_Scwrl_3MTI.pdb: