Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKILVTGAAGFIGSHLCEELLKDKKHNVIGIDDFIGPTPFSLKLKNLKNLLPEKRFTFIKENLLTADLASLLEGVDVIFHLAAIPGVRSSWGNHFHPYAAHNIQALQRLLEACREHSIQTFVFASTSSVYGEKQGK-VSENTSLSPLSPYGVTKLTGEKLCHVYKQSFGIPIVILRFFTVYGPRQRPDMAFHRLIKQHLQQ-KPLTIFGDGQQSRDFTYISDCVKGITAVLGKPH---LIGETVNIGGAERASVLKVVSLIEDISGRKATLHF-SDKIAGEPSNTWADISKAKQLLHYDPATSLKDGLTNEIAYLSSLYQGE 3KO8 Chain:A ((1-310)) MRIVVTGGAGFIGSHLVDKLVEL-GYEVVVVDNLSSGRR---E-------FVNPSAELHVRDLKDYSWGAG-IKGDVVFHFAANPEVRLSTTE-PIVHFNENVVATFNVLEWARQTGVRTVVFASSSTVYGDADVIPTPEEEPYKPISVYGAAKAAGEVMCATYARLFGVRCLAVRYANVVGPRLRHG-VIYDFIMKLRRNPNVLEVL----QRKSYLYVRDAVEATLAAWKKFEEMDAPFLALNVGNVDAVRVLDIAQIVAEVLGLRP-EIRLV----GDVKYMTLAVTKLMKLTGWRPTMTSAEAVKKTAEDLAKEL---

General information: TITO was launched using: RESULT: Template: 3KO8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1762 -101875 -57.82 -351.29 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -57.82 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_3KO8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KO8-query.scw PDB file : Tito_Scwrl_3KO8.pdb: