Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPINSFENYPMSWKPSIDKAEKPIYKALAGQLEQDILNGVLLPGTKLPPQRELADYLDLNVSTISKAFKVCELKGLLSATVGSGTFVSYDALSNAYLLEDTKPTHLIEMGAILPDHA----SYEPLLYQLKNMVHEEDYEKWFSYGRAGESLWQKDAAVKLIQR-GGF-----ETSVDHILFANGGQNAIAATLASLCKPGDRIGVDHHTYPGLKTVASLLSVQIVPIKSENDE---MSPESLEYACKNENIKGLYLIPDYHNPTTSFMSVENRRMVADIVKKYNLFVIEDASYHLLNKNP--LPALASFA-PQQVIHIASLSKSLA-PGLRLAYVAVPR-Q-----YKEPISKALYNLNITVSPLLAELTARTIVSNQFEVLIESHREQTIRRNQLVNRYVAGY---TCLGVETGIFRWLLLPGKM-----SGAEFEELAA-RLGVQVYAAERFVVGNSCPERAVRVSVCAPKTLEELEQGLMILRRLLDDLT 4IX8 Chain:A ((36-442)) ------------------------------------------------SFRRIAS-----SKHAQRTLQPLN--NLT--MKPS----------R-------STKSNLRLSIGDPTVDGNLKTPDIVTEAMVDVVRS---GKFNGYPPTVGADNLRQVVSTYWRRFCQTKSRQEALKWENVIITSGVSQAIVLALTALCNEGDNILVCAPSFPHYKSVCDSYGIECRYYYLDPSKSWECDLRAAAGMVD-SHTKAFVII-NPSNPCGSNFSRAHVSDIIDFCQQHQIPLISDEIYAEMVLNNGIFTSVADFDTNVPRLILGGTAKYQVCPGWRVGWSILIDPMNVAGDWAVGMERLTQ-LIAGVNSICQEAIARTLLKCP-TECTEHIVTQLEAGAKVYARLLEHDIGISMEAPQASMFVMLKLNLSYFQDLKSDMEFYEKLLDEENVQVLPGEIFGM-----SGFLRATVSR--PSAVLNEAVDRIIEFCERH-

General information: TITO was launched using: RESULT: Template: 4IX8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2012 -236686 -117.64 -634.55 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -117.64 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_4IX8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IX8-query.scw PDB file : Tito_Scwrl_4IX8.pdb: