Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METFKSILHEAQRIVVLTGAGMSTESGIPDFRSAGGIWTE----DASRMEAM-SLDYFLSYPRLFWPKFKELFQMKMSGSFEPNEGHLLLAELEKQGKQVDIFTQNIDGLHKKAG--SRHVYELHGSIQTAACPACGARYD----LPHLLEREVPECTAAGNNGDICGTVLKTDVVLFGDAV-MHFDTLYEKLDQADLLLVIGTSLEVAPARFVPEDASLIPGMKKVIINLEPTYCDSLFDMVIHQKIGEFARSLGMKK 4V1C Chain:A ((18-206)) -----------QRVVVMVGAGISTPSGIPDFRSP---YSNLQQYDLPYPEAIFELPFFFHNPKPFFTLAKELY----PGNYKPNVTHYFLRLLHDKGLLLRLYTQNIDGLERVSGIPASKLVEAHGTFASATCTVCQRPFPGEDIRADVMADRVPRCP-------VCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSLEVEP-------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4V1C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 791 -72655 -91.85 -419.97 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -91.85 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_4V1C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4V1C-query.scw PDB file : Tito_Scwrl_4V1C.pdb: