Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MHRGKGMKFVGDSRI-PAEKKPNIPKDYSEYPGKTEAFWPNFLLKEWMVGAVFLIGFLVLTIVH--QPPLERMADPTDTGYIPLPDWYFLFLYQLLKYEYAAGSF-----------TVVGAMIMPGLAFGALLLAPFLDRGTERRPWKRPVAVGMMLLAISAAVFLTWQSVATHDWAKAEEQGKITKE-------------------ADIDTNAEGYKVFKEQ----------GCISCHGDNLQ-------------------GGAAGPSLVDSGLKPDEI---KKIAVEGKGK---------------MPAGVFKGNDKQLEELAKFISETTAK 2Y38 Chain:A ((8-398)) GFSLHPPYFNLAEGARITASATCHPVSNAIDGTERWWQSPPLSRGLEYNEVNVTLDLGQVFHVAYVLIKFANSPRPDLWVLERSTDFGHTYQPWQFFASSKRDCLERFGPRTLERITQDDDVICTTEYSRIVP-LENGEIVVSLVNGRPGALNFSYSPLLRDFTKATNIRLRFLRTNTLLGHLMGKALRDPTVTRRYYYSIKDISIGGRCVCHGHADVCDAKDPLDPFRLQCACQHNTCGGSCDRCCPGFNQQPWKPATTDSANECQSCNCHGHAYDCYYDPEVDRREASQNQDNVYQGGGVCLDCQHHTTGINCERCLPGFFRAPDQPLDS-PHVCRPAAAH

General information: TITO was launched using: RESULT: Template: 2Y38.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1293 31639 24.47 125.05 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 24.47 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.130

(partial model without unconserved sides chains): PDB file : Tito_2Y38.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y38-query.scw PDB file : Tito_Scwrl_2Y38.pdb: