Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDMKHTLLEALGIEIVENTAERCVAVMPVDHRTVQPFGYLHGGASVALAETAASAGAQNLIDHTTQACVGLEINANHLKSVKEGTVKAIAEPVHIGRTTIVYHIHIYDEQERLICISRCTLAVIKK 4K4C Chain:B ((18-136)) ----NTMVAHLGIVYTRLGDDVLEAEMPVDTRTHQPFGLLHGGASAALAETLGSM-AGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVCQPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVL--

General information: TITO was launched using: RESULT: Template: 4K4C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 588 -82946 -141.06 -697.03 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -141.06 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.532

(partial model without unconserved sides chains): PDB file : Tito_4K4C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K4C-query.scw PDB file : Tito_Scwrl_4K4C.pdb: