Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKQTIRVELTSTKKPKPDPNQLSFGRVFTDHMFVMDYAADKGWYDPRIIPYQPLSMDPAAMVYHYGQTVFEGLKAYVSEDDHVLLFRPEKNMERLNQSNDRLCIPQIDEEQVLEGLKQLVAIDKDWIPN-AEGTSLYIRPFIIATEPFLGVAASHTYKLLIILSPVGSYYKEGIKPVKIAVESEFVRAVKGGTGNAKTAGNYASSLKAQQVAEEKGFSQVLWLDGIEKKYIEEVGSMNIFFKINGE---IVTPMLNGSILEGITRNSVIALLKH-WGLQVSERKIAIDEVIQAHKDGILEEAFGTGTAAVISPVGELIWQDE-TLSINNGETGEIAKKLYDTITGIQKGAVADEFGWTTEVAALTESK 3UYY Chain:B ((22-358)) ---------------ADIDWSTLGFSYIRTDLRYLAHWK-DGEWDAGTLTEDNQIHLAEGSTALHYGQQCFEGLKAYRCADGSINLFRPDQNAARMRMSCRRLLMPELSDEQFIDACLQVVRANEHFLPPYGTGGSLYLRPFVIGVGDNIGVRTAPEFIFSVFCVPVGPYFKGGLTPTNF-ITSDYDRAAPHGTGAAKVGGNYAASLLPGYEAKKRDFADVIYLDPATHTTIEEAGAAN-FFAITQDGQKFVTPQ-SPSILPSITKYSLLWLAEHRLGLEVEEGDIRIDEL------GKFSEAGACGTAAVITPIGGIQHGDDFHVFYSESEPGPVTRRLYDELVGIQYGDKEAPEGWIVKV-------

General information: TITO was launched using: RESULT: Template: 3UYY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1826 -76331 -41.80 -230.61 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -41.80 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_3UYY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UYY-query.scw PDB file : Tito_Scwrl_3UYY.pdb: