Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNYSRIALFG--GFAAAALLAGCSASSGKPGGKDWTRWTCDSKAVIEWRYIDGSKNIVDLRLKDEDDVVHHLEQEPAAVGAFYSDGRLGFHLENDEGLVYWVETD-DLIGRGCKAR------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ 2NIP Chain:A ((1-286)) -AMRQCAIYGKGGIGKSTTTQNLVAALAEMG-KKVMIVGCDPKADSTRLILHSKAQNTIMEMAAEAGTVEDLELEDV--------------LKAGYGGVKCVESGGPEPGVGCAGRGVITAINFLEEEGAYEDDLDFVFYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAMYAANNISKGIVKYANSGSVRLGGLICNSRNTDREDELIIALANKLGTQMIHFVPRDNVVQRAEIRRMTVIEYDPKAKQADEYRALARKVVDNKLLVIPNPITMDELEELLMEFGIMEVEDESIVGKTA

General information: TITO was launched using: RESULT: Template: 2NIP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) 8446 for 615 contacts (13.7/contact) + 2D Compatibility (PS) -10236 + (NN) -4568 + (LL) 572 1D Compatibility (HY) -3200 + (ID) 1200 Total energy: -10186.0 ( -16.56 by residue) QMean score : 0.076

(partial model without unconserved sides chains): PDB file : Tito_2NIP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NIP-query.scw PDB file : Tito_Scwrl_2NIP.pdb: