Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGDSILFTVKNEHMALITLNRPQAANALSAEMLRNLQMIIQEIEFNSNIRCVILTGTGEKAFCAGADLKERIKLKEDQVLESVSLIQRTAALLDALPQPVIAAINGSALGGGLELALACDLRIATEAAVLGLPETGLAIIPGAGGTQRLPRLIGRGKAKEFIYTGRRVTAHEAKEIGLVEHVTAPCDLMPKAEELAAAISANGPIAVRQAK---FAINKGLETDLATGLAIEQKAYEQTI-------PTKDRREGLQAFQEKRRAVYKGI 3R0O Chain:B ((24-271)) ------------NVALITIDRPDARNAVNGAVSTAVGDALEEAQRDPEVWAVVITGAGDKSFCAGADLKA-ISRGENLYHAEHPEWGFAGYVHHFIDKPTIAAVNGTALGGGSELALASDLVIACESASFGLPEVKRGLIAGAGGVFRIVEQLPRKVALELVLTGEPMTASDALRWGLINEVVPDGTVVEAALALAERITCNAPLSVQASKRVAYGADDGI-------IGAEEPKWERTIREFTELLKSEDAKEGPLAFAEKRQPVWK--

General information: TITO was launched using: RESULT: Template: 3R0O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1271 -120620 -94.90 -506.81 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -94.90 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_3R0O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3R0O-query.scw PDB file : Tito_Scwrl_3R0O.pdb: