Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMEKTFIMVKPDGVQRQLIGDILSRFERKGLQLAGAKLMRVTEQMAEKHYAEHQGKPFFGELVEFITSGPVFAMVWEGENVIEVTRQLIGKTNPKEALPGTIRGDYGMFVGKNIIHGSDSLESAEREINIFFKNEELVSYQQLMAGWIY 3BBF Chain:C ((3-150)) -LERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAHDWVY

General information: TITO was launched using: RESULT: Template: 3BBF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 680 -72243 -106.24 -488.13 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain C : 0.88 3D Compatibility (PKB) : -106.24 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_3BBF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3BBF-query.scw PDB file : Tito_Scwrl_3BBF.pdb: