Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAVTYQG-IKNVVVKDVPDPKIEKSDDMIIKVTSTAICGSDLHLIHGFIPNMQEDYVIGHEPMGIVEEVGSGVTKLKKGDRVIIPFNIACGECFFCKNQLESQCDQSND-NGEMGAYFGYSG--------QTGGYPGGQAEYLRVPFANFTHFKIPESCEEPDEKLSVIADAMTTGFWSVDNA-GVKKGDTVIVLGCGPVGLFAQKFCWLKGAKRVIAVDYVNYRLQHAKRTNKVEI-VNFEDHE--NTGNYLKEITKGGADVVIDAVGMDGKMSDLEFLASGLKLHGGTMSALVIASQAVR-KGGTIQITGVYGGRYN-GFPLGDIMQRNVNIRSG-QAPV--IHYMPYMFELVSTGKIDPGDVVSHVLPLSEAKHGYDIFDSKMDDCIKVVLKP 2FZW Chain:A ((7-372)) CKAAVAWEAGKPLSIEEIEVA-PPKAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHLGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVA--DISVAKIDPL--APLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEF-GATECINPQDFSKPIQEVLI-EMTDGGVDYSFECIGNV--------------------KVMRAALEACHKGWGVSVVVGVAASGEEIATRP-FQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFELMHSGK--SIRTVVK-

General information: TITO was launched using: RESULT: Template: 2FZW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2150 -41452 -19.28 -119.46 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -19.28 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.447

(partial model without unconserved sides chains): PDB file : Tito_2FZW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FZW-query.scw PDB file : Tito_Scwrl_2FZW.pdb: