Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEELLERVFSFSDVDKLIDFISYELQKPVILESADFFLLAYNSYYINHFDSANQQTIFSKKCPVQIFERFLKDGIIEKLKTEPEPFRVNKIESIGLNQRVVVSAKHKGEVMGYIWIQELDQNLTDEELDFLYETSFHVGKIIYKTNKLKQEKEEKAEDLIK--RAIYQQFTSEKELRREAERINTVLPSMFSVVILHAANGDGEAVEDLKENIRSYLNLRDKVSHVLTIESNIVIVVASFSQKSSVSSAASEFINKLLTHFHFQKIPTPIYIG---IGNEYNHLLKLGKSYTEALEVIKAAEITGNQENIPYEYA-KLGIYRYLESIEQKNEFLEYENKDLALLKAKDEESSTELLKTLEIYLLNNCKTKPAAEQLFIHQNTLNYRIKQITEMTSIDLSDFRTRCQLYLDLMLMKKK 5BYO Chain:A ((15-225)) --------------------------------------------------------------------------------------------------KLAKAAYYSGTTVEEAYKLALKLGISVEELLKLAEAAYYSGTTVEEAYKLALKLGISVEELLKLAKAAYYSGTTVEEAYKLALKLGISVEELLKLA--KAAYYSGTTVEEAYK-----LALKLGIS----VEELLKLAEAAYYSGTTVEEAYKLALKLGISVEELLKLAKAAYYSGTTVEEAYKLALKLGISVEELLKLAKAAYYSGTTVEEAYKLALKLGI-------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5BYO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 977 -77744 -79.57 -379.24 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -79.57 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.290

(partial model without unconserved sides chains): PDB file : Tito_5BYO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BYO-query.scw PDB file : Tito_Scwrl_5BYO.pdb: