Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAQSSFLQLNSIFISILIEAIPFILIGVILSGIIQMFVSEEMIARIMPKNRFLAVLFGALAGVLFPACECGIIPITRRLLLKGVPLHAGVAFMLTAPIINPIVLFSTYIAFGNRWSVVFYRGGLALAVSLIIGVILSYQFKDNQLLKPDEPGHHHHHHGTLLQKLGGTLRHAIDEFFSVGKYLIIGAFIAAAMQTYVKTSTLLAIGQNDVSSSLVMMGLAFVLSLCSEVDAFIASSFSSTFSLGSLIAFLVFGAMVDIKNLLMMLAAFKKRFVFLLITYIVVIVLAGSLLVKG 1RH5 Chain:B ((29-53)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------TKDEYLAVAKVTALGISLLGIIGYI-------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1RH5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 7 -304 -43.36 -12.14 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain B : 0.39 3D Compatibility (PKB) : -43.36 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.39 QMean score : 0.287

(partial model without unconserved sides chains): PDB file : Tito_1RH5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RH5-query.scw PDB file : Tito_Scwrl_1RH5.pdb: