Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRRVETITFDGGTLEYSVTGKGTPILVMHGGH------SNCYEEFGYTALIEQGYSIITPSRPGYGRTSKEI---GKSLANACRFYVKLLDHLQIESVHVIAISAGGPSGICFASHYPERVNTLTLQSAVTKEWLTPKDTEYKLGEILFRP-PVEKWIWKLISSLNNAFPRLMFRAMS-PQFSTLP-----FQRIKSLMNEKDIEAFRKMNSRQRSG-EGFLIDLSQTAAVSLKDLQA----IICPVLIMQSVYDGLVDLSHAHHAKEHIRGAVLCLLHSWGHLIWLGKE-AAETGSILLGFLES 2RI6 Chain:A ((11-280)) ---KINEKGFSDFNIHYNEAGNGETVIMLHGGGPGAGGWSNYYRNVG--PFVDAGYRVILKDSPGFNKSDAVVMDEQRGLVNA-RAVKGLMDALDIDRAHLVGNAMGGATALNFALEYPDRIGKLIL--------MGPG----GLGPSMFAPMPMEG-------------IKLLFKLYAEPSYETLKQMLQVFLYDQSLITEELLQGRWEAIQRQPEHLKNFLIS-AQKAPLSTWDVTARLGEIKAKTFITWGRDDRFVPLDHGLKLLWNIDDARLHVFSKCGH--WAQWEHADEFNRLVIDFL--

General information: TITO was launched using: RESULT: Template: 2RI6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1419 -141926 -100.02 -572.28 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -100.02 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.393

(partial model without unconserved sides chains): PDB file : Tito_2RI6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RI6-query.scw PDB file : Tito_Scwrl_2RI6.pdb: