Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGENMFKKEKVTEYIWTILIPTIITFIISWVGSYYNGTSTVSIGQPTKVSGQYITPINISPYHDIKELRITFPQKLDVKQISSNEPINVKSDKNNIGVESNSTFEIAKIVENNSVQLLITTQKKLNDKEIRIDKNGNNISVNYESQIVNPAKKQLINLIITSSIYFIMLNILALIMNKRWDKYYAKMKNEIKEFEDNAKDLDKKSKKKSEELSELRKTLNQAFEETDRIKYHEKKKQILLLAKLNDYKKELTFWRNTIRKVLYELPDGDKKADKLIGTVTSSLKTYGTVEKNEHDYESLKVAAALLNDSDKRS 3G7B Chain:A ((42-160)) ----------------------------------------------------------------------------------------------------------------NQIEVVIE-----KDNWIKVTDNGRGIPVDIQEKMGRPA----VEVILTSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF-----DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLN--------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3G7B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 452 -14422 -31.91 -121.19 target 2D structure prediction score : 0.29 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -31.91 2D Compatibility (Sec. Struct. Predict.) : 0.29 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.073

(partial model without unconserved sides chains): PDB file : Tito_3G7B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G7B-query.scw PDB file : Tito_Scwrl_3G7B.pdb: