Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDFAFKLYEYNVWANQQIFNRLKELPKEIYHQEIQSVFPSISHVLSHVYLSDLGWIEVFSGKTLSDALALAEQLKEQTEAKEIEEMEDLFLRLSERYILFLQQKEQLNKPLQIQNPSSGIMKTTVSELLPHVVNHGTYHRGNITAMLRQAGYASAPTDYGLYLFMTKTEKA 3L49 Chain:A ((24-114)) -------------------------------------------------YQAQIAEIERLGGT----AIALDAGRNDQTQVSQIQT---LIAQKPDAIIEQLGNLDVLNPWLQKIN-DAGIPLFTVDTATPHAINNTTSNNYSIGAEL------------------------

General information: TITO was launched using: RESULT: Template: 3L49.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 293 -16575 -56.57 -182.14 target 2D structure prediction score : 0.29 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -56.57 2D Compatibility (Sec. Struct. Predict.) : 0.29 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.213

(partial model without unconserved sides chains): PDB file : Tito_3L49.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3L49-query.scw PDB file : Tito_Scwrl_3L49.pdb: