Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTVLILNFPAEGHVNPTLGITKAFSDKGYDVHYISTEKYKKRLEAAGATVHLHRDLLR---TTPIHVGSPNGILDFVKIHIKTSLDILQIVKDLSKSIQFDFVYYD--KFGAGELVRDYLDIPGVSSSASFLFGE-------EHLKILPLHPESGAPLELDQEC---EDLLAKMKETYGVAPKNLVQFMNNKGELNVVYTSRYFQPESDRFGDEC-LFIGPSFPKRAEKTDFPIEQLKDEKVIYISMGTVLDHTEDFFNLCIDAFSGFNG-KVVIAAGEKADLTKLKQAPENFIIAPYVPQLEVLEQSDVFITHGGMNSVNEGIHFSVPLVVMPHDKDQPMVAQRLSELHAGYVISKDEVNAQILKQAVDEVLRNDQYTAGIKKINQSFKECMDMEEVMERIDELIRQKNK 4M60 Chain:A ((13-381)) ---IAMFSIAAHGHVNPSLEVIRELVARGHRVTYAIPPVFADKVAATGARPVLYHSTLPGPDADPEAWGS--TLLDNVEPFLNDAIQALPQLADAYADDIPDLVLHDITSYPARVLARRW-GVPAVSLSPNLVAWKGYEEEVAEPMWREPRQTERGRAYYARFEAWLKENGITEHPDTFASHPPR-----------SLVLIPKALQPHADRVDEDVYTFVGACQG-------W-QRPAGAEKVVLVSL-------PAFYRECVRAFGNLPGWHLVLQI-----PAELGELPDNVEVHDWVPQLAILRQADLFVTHAGAGGSQEGLATATPMIAVPQAVDQFGNADMLQGLGVARKLATEEATADLLRETALALVDDPEVARRLRRIQ-------------------------

General information: TITO was launched using: RESULT: Template: 4M60.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1663 -212474 -127.77 -638.06 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -127.77 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.397

(partial model without unconserved sides chains): PDB file : Tito_4M60.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M60-query.scw PDB file : Tito_Scwrl_4M60.pdb: