Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METIIIALIAFIIGIIAIPIVLFAWIYIKDEKQQEHSILRNYPVIGRFRYILEKIGPELRQYLYSNDNEEQPFSRKEYEQTVISGKYKSRMMGFGSVRDFDKPGYYIRNAMFPKQREEMHVNQTPKIETQIYKMDADNLFKRKEHAEHIKADPYFLHPDDVQVIGEHTCEKPFYVKGLVGQSAMSYGSLGERAVTALSKGLHLAGGTWMNTGEGG--------LSEYHLKGGADIICQIGPGLFGVRKRNGEFSWEEFKRKSR---------IDQIKAFELKLAQGAKT-RGGHVDGAKVSEEVADIRNVEPGKSIDSPNRFYEFSNPPEMLDFIEKLRDVG-QKPVGIKLVAGHPEELHELFSHMQKSGKHPDFITIDGSEGGTGASFYELADTVGLPIMTALPIVDTLLKQYGLRSQLKIFASGKLLTPDKIAVALALGADFVNIARGMMFSVGCIRALVCHTNTCPAGVATTDPKLQKALSVEEKQHRVCNYVISLREGLFNLAAAAGINSPIHFSKEHVVYRKEDGSIINIDNLIHQFVS 1OFE Chain:A ((875-1192)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MSLGALSREAHETLAIAMNRLGAK-SNSGEGGEDVVRYLTLDDVDSEGNSPTL----PHLHGLQ--NGDTANSAIKQIASGRFGVTPEYLMSGKQLEIKMAQGAKPGEGGQLPGKKVSEYIAMLRRSKPGVTLISPPPHHDIYSIEDLAQLIYDLHQINPEAQVSVKLVA--EIGIGTIAAGVAKA--NADIIQISGHDGGTGASPLSSIKHAGSPWELGVTEVHRVLMENQLRDRVLLRADGGLKTGWDVVMAALMGAEEYGFGSIAMIAEGCIMARVCHTNNCPVGVATQQERLRQRFKGVPGQ--VVNFFYFIAEEVRSLLAHLGYRS------------------------------

General information: TITO was launched using: RESULT: Template: 1OFE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1666 -88522 -53.13 -296.06 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -53.13 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.358

(partial model without unconserved sides chains): PDB file : Tito_1OFE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1OFE-query.scw PDB file : Tito_Scwrl_1OFE.pdb: